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Additionally, it must be noted that an individual biomarker, for example, sKlotho, inherently has some limitations for accurate diagnosis and monitoring progression in CKD without the combination of a set of other biomarkers because a single indicator lacks sufficient performance and efficiency to reflect the complexity of the mechanisms underlying CKD pathogenesis and development

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The effect ( fa ) on inhibiting intracellular ROS production was expressed using the following equation: Where F max is the fluorescence intensity of the positive control, F min is the fluorescence intensity of the negative control, and F sample is the intracellular fluorescence intensity of the sample

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