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The supernatants (100 g protein) were mixed with 100 mM Tris-HCl containing 5 mM EDTA, 0.1% Triton X-100, 1.5 mM NADPH, 3 mM GSH, and 10 M 1-SA-2-15-HpETE-PE, in the absence or presence of 10 M RSL3, and incubated at 37C for 30 minutes
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