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Quantification was based on the molar extinction coefficient of NADPH, which is 6.22 mM/cm
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Yanzhang et al., 2025)
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Targeted Therapy for the Central Nervous System 369403:2025 J
As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins
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