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While the effects of a vitamin B12 injected will become noticeable around 24 hours after administration, they will start to take effect almost immediately and will stay in your system for around 1 week, but may vary depending on the metabolic rate of the patient

Choline, for example, is involved in lipid transport and metabolism (a vital part of energy storage), cell membrane signaling, and nervous system function

Specifically, selenium is required for the enzyme glutathione peroxidase to function

PMID 3053703

This creates a challenging bottleneck for formulators who need to deliver a functional dose to achieve the desired output

Oaklander AL, Mills AJ, Kelley M, et al