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In an Italian family where several members are obese it was found that a missense mutation was present in their CART gene
A single colony was inoculated into 5 ml of Tryptic Soy Broth (BD Difco) under anerobic conditions at 37 C for an overnight culture lasting 3 days
GLP-1 receptor agonists bind to GLP-1 receptors in the pancreas, stimulating insulin secretion in a glucose-dependent manner whilst suppressing glucagon release
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