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[13] [19] Specifically, the function of GSTs in this role is twofold: to bind both the substrate at the enzyme's hydrophobic H -site and GSH at the adjacent, hydrophilic G-site, which together form the active site of the enzyme

Syringe handling: Use a separate syringe for each compound when running separate vials

Over-dilution below 10mg/mL increases oxidative exposure

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The addition of reduced glutathione causes turbidity that disappears after the addition of sodium hydroxide, yielding a clear solution