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p = 0.59 for interaction between subjects with prior MI or stroke vs no prior MI or stroke and p = 0.93 for interaction between subjects with established CVD vs CV risk factors only)

The COs were fixed with a primary fixation buffer (1% glutaraldehyde in 0.1 M phosphate buffer and 4% paraformaldehyde) for 2 h at room temperature and changed to fresh fixation buffer for overnight incubation at 4 C

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