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PMID 11352721
p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells
In this study, the placebo was normal saline (salt water) applied after the same radiofrequency procedure, allowing researchers to isolate the effect of the active serum ingredients
Not misusing nitrous oxide
It also promotes angiogenesis, or the formation of new blood vessels, allowing for increased oxygenation to the tissues
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