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Preparation : No special preparation is needed

Your B12 shot frequency matters so much because B12 is water-soluble, so any excess is excreted within hours

Determine Concentration (example: 10 mg vial 1 mL water = 10 mg/mL): Concentration (mg/mL) = Total Peptide (mg)Total BAC Water (mL) Calculate Dosage Volume (example: 1 mg dose 10 mg/mL = 0.1 mL): Volume to Draw (mL) = Desired Dose (mg)Concentration (mg/mL) Convert to Syringe Units: Using a standard U-100 (1 mL/100 unit) insulin syringe, 0.1 mL equals 10 units

doi: 10.1056/NEJMoa0907118

The substantially reduced IGFBP affinity makes IGF-1 DES a precise tool for in vitro studies requiring direct IGF-1R activation without IGFBP-mediated sequestration confounding the experimental readout

Wang, L., et al