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The reaction mixtures were manually mixed using a 100 to 300 l multichannel pipette (set to 250 l) and the microplate was inserted into a BioTek H1 Synergy microplate reader equilibrated to 37 C and vigorously shaken for 1 min followed by a 3.5-min delay

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The effects of valproic acid neurotoxicity on aggressive behavior in zebrafish autism model

Most protocols target the middle of this range