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Research from the Journal of Clinical Endocrinology & Metabolism confirms that fasted, nocturnal GH secretagogue administration produces the most reliable amplification of endogenous pulsatile secretion patterns
Plasmids of wildtype and mutants of ARE motifs were transfected to K562 (#CRL-3344, ATCC), an erythroleukemia cell, along with pDsRed1-N1 (PT3405, Clontech), which is used as a transfection efficacy control
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