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We engineer a melatonin-inducible gene switch consisting of ectopically expressed MTNR1A linked to an amplifier module utilizing the native G s protein-mediated cell signaling cascade, which involves adenylyl cyclase, cAMP, protein kinase A and the cAMP-responsive transcription factor CREB, to drive transgene expression from a synthetic promoter
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instead, swirl it in a gentle manner
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