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Isolation of intestinal crypts in mice (1) Fast mice overnight or for 24 hours, then euthanize 5 to 8-week-old mice using carbon dioxide asphyxiation

injections of GLP-1:GIP + Lani, GLP-1:GIP:Lani or vehicle for 14 days

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Add 2ml BAC water concentration = 2.5mg/ml (2,500mcg/ml)

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