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It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile

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Gene-Expression Claims Require Direct Evidence Acute signaling and gene-expression adaptation occur on different time scales

This was seen in mice that were treated with 5 Amino 1 MQ, in which large-sized myofibers were observed as compared to the controls