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The histological analysis showed that DU-145 xenografts, exposed to ALCAR, had less cellularity as compared to untreated ones (Additional file 1: Figure S4A)

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On the other hand, an undue focus on optimizing the C-3 position may come at the expense of other pharmacophore regions such as the rigid steroidal core and the C-20 keto group, which are also important structural stability and target binding determinants, and have not been investigated deeply either

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