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Isolation of intestinal crypts in mice (1) Fast mice overnight or for 24 hours, then euthanize 5 to 8-week-old mice using carbon dioxide asphyxiation
Presented for research literature review only Kong YX et al
Sci Rep 9 , 13909 (2019)
The lEVs and sEVs were further purified by density gradient centrifugation, and the low-density fraction of 1.116 g/ml and the high-density fraction of 1.176 g/ml were collected, respectively
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