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Glutathione levels and BAX activation during apoptosis due to oxidative stress in cells expressing wild-type and mutant cystic fibrosis transmembrane conductance regulator

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Cells were washed with Hanks Balanced Salt Solution (Lonza) buffered with 20 mmol/l HEPES (Sigma) and supplemented with 0.1% BSA (Sigma) (wt/vol.)

Consistency over intensity is the goal