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Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin
Although vitamin B12 is naturally found in animal products such as meat, dairy, and eggs, some people may be unhappy with a vitamin B12 deficiency due to diet, health, or age
Vitamin C is also a potent antioxidant which protects the cells from the free radical damage that causes premature aging
Reduced FcRn-mediated transcytosis of IgG2 due to a missing glycine in its lower hinge
This pathophysiological cascade is now accepted to be of major relevance for the development of metabolic diseases including type II diabetes, cardiovascular diseases and non-alcoholic fatty liver disease (NAFLD) or non-alcoholic steatohepatitis (NASH) [88]-[93]
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