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After centrifugation (12,000 x g for 10 minutes, 4C), supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100)
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MR-PRESSO detected outliers in the overall associations with CAD in FinnGen (shown in Additional file 1: Table S5), where we used corrected estimates from MR-PRESSO
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