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Then, 10 l aliquots of these reactions were directly added to 90 l ultrafiltered flavan-3,3,4-triol ( 4 ) to initiate the second step of the two-step in vitro assays with 3,4- cis -leucocyanidin ( 6 ) as described below
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Molecular Metabolism
The increase in PyroGlu could be due to three key points: (I) the catabolism of GSH through gamma-glutamyl transpeptidase, (II) and/or to a limiting factor in the second step of gamma-glutamylcysteine synthetase (III) or a limiting factor in the following step involving glutathione synthetase
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