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The LC gradient program was: 98% B for 1 min, followed by a linear decrease to 40% B within 5 min, then maintain 40% B for 13 min, then return to 98% B in 1 min and finally 5 min at 98% B for column equilibration

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Sandoval DA, DAlessio DA (2015) Physiology of proglucagon peptides: role of glucagon and GLP-1 in health and disease

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