The FDA's 2024 review found no published human pharmacokinetic study that formally characterized the subcutaneous route, so these amounts are anecdotal rather than an approved clinical protocol
What is vitamin B12 (cyanocobalamin) used for
What is missing There is no published human pharmacokinetic study of subcutaneous AOD-9604, no published human topical or intranasal exposure data, no Phase 3 efficacy trial, no long-term safety data beyond 24 weeks, and no published human cartilage or joint outcome data
Pyridoxine requirements are also increased during lactation
The Evidence that oral supplementation can be used to effectively treat Pernicious Anaemia is weak
Being honest about that gap is not a weakness of this guide it is the accurate picture, and it is why every dosing figure here is framed as a research protocol rather than a recommendation