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The 5x half-life rule By the time five half-lives have passed, only about 1 to 3 percent remains, which is considered negligible

[2] Spreckley, E., & Murphy, K

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This might indicate total changes in hormone secretion to be too minor to be recovered in serum due to the limited tissue affected

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The process as claimed in claims 1 to 6, wherein said base used for deprotection is selected from the group consisting of tert-butyl amine, 20% of 4-methyl piperidine in Dimethyl formamide, 20% of piperidine in Dimethyl formamide and 20% of piperazine in Dimethyl formamide