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Cells were then incubated overnight at 4C with different pairs of the following antibodies: mouse anti H2AX (Millipore cat.# 05-636), rabbit anti ICP8 antibody (kindly provided by prof WT Ruyechan, Univesity of Buffalo, Buffalo, NY, USA), as primary antibodies and Cy2-conjugated donkey anti-rabbit IgG and Alexa Fluor 568 donkey anti-mouse IgG (Molecular Probes, Life Technologies), as secondary antibodies (30 min at room temperature)
It is a synthetic pentapeptide composed of both standard and modified amino acids
The extract was clarified by centrifugation (17,000 g for 20) and then diluted 1:10 into post-lysis buffer (10 mM MgCl 2 , 10 mM Zn(II) diacetate, 1 M Tris pH 8.0)
Our extensive experience in peptide synthesis and handling informs our recommendations
