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The gel was stained in Coomassie staining solution for overnight followed by destaining in milliQ water and the results, presented in Figure S3 show, that all commercial proteins used have high purity and are suitable for metal-binding studies

(6) These spikes have been suggested to reach up to 26.6 ng/ml, which is more than double the amounts of 5-10 ng/ml that are typically observed in physiological conditions

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