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The pairing is a frequently studied model system in pre-clinical somatotroph research where two complementary upstream pathways converge on the same pituitary cell population

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While glutathionylation of roGFP2 C208S also caused an increased fluorescence at an excitation of 400 nm and a decreased fluorescence at an excitation of 484 nm, glutathionylation of roGFP2 C151S resulted in a decreased fluorescence at both excitation wavelengths

These effects develop gradually over weeks to months of consistent use

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To isolate nanoparticles, the suspension was centrifuged at 17000 xg for 40 minutes at 4 degrees Celsius