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Association study of glutathione S-transferase P1 (GSTP1) with asthma and bronchial hyper-responsiveness in two German pediatric populations

Colonies with recombinant bacmids were selected on kanamycin/tetracycline/gentamycin plates by blue-white selection, and the presence of the Coeae6g coding sequence was verified with PCR (pUC/M13 Forward -M13 Reverse primer pair) and via Sanger sequencing (GENEWIZ, Azenta Life Sciences, Germany), using primers pUC/M13 Forward and M13 Reverse, and Coeae6g Fin and Rin (Supplementary Data S7)

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This study demonstrated that, although the respiratory failure was as a leading contributor to mortality in patients with PWS, the survival estimates for patients with PWS have increased since 2000, especially for fatal cardiac events in females and thrombotic and gastrointestinal-related mortality, most likely for earlier diagnosis and proactive interventions to prevent very severe obesity [38]

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