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On each gel, a size marker was run: 3 l PageRuler (Thermo Fisher Scientific, 26616) or 3 l PageRuler plus (Thermo Fisher Scientific, 26620) and 17 l 2 Laemmli buffer
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Structurally, they differ considerably
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The rubber stopper is exposed to the open air, and failing to sanitize it is the most common way to introduce bacteria into your sterile solution.
Cited preclinical work covers rat Achilles tendon transection, medial collateral ligament injury, segmental bone defects and gut-injury models
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