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The cells were maintained in RPMI/B27 plus CHIR99021 for the first 24 hours, followed by its removal and re-feeding with just RPMI/B27 (with or without insulin) and maintained for a further 24 hours
It's also unlikely to cause the "eye sting" some chemical filters are known for
Most of the bodys carnitine is located within skeletal muscles, where it is crucial for the supply of energy by the beta-oxidation of fatty acids
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