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It escalates
Serum components can interact with MT-1 and reduce apparent potency at lower working concentrations
The temperature was subsequently controlled by a constant-temperature heating plate, the pH was maintained by acidbase titration, the anaerobic environment was ensured by nitrogen bubbling, basic chemical culture medium was provided to supply nutrition to simulate the intestinal ecological conditions, and the experiment was terminated when the substrate was exhausted and toxic metabolites (such as ammonia and organic acids) accumulated, destroying the initial flora balance (Nissen et al., 2020)
The activities of nnAbs against free virions could, in principle, involve two types of target
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