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The probe plate was then prepared with mitochondrial inhibitors: 25 l 5 M rotenone (Sigma-Aldrich, 45656) and 5 M antimycin A (Sigma-Aldrich, A0149)

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Further analyses have revealed pronounced gut microbial dysbiosis in cirrhotic patients with hepatic encephalopathy (HE), marked by increased abundances of bacterial families such as Alcaligenaceae, Porphyromonadaceae, and Veillonellaceae, as well as genera including Enterococcus , Megasphaera , and Burkholderia (498)