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Primary antibodies against FOXO4 (Cell Signaling Technology 9472) and TP53BP1 (BD Biosciences 612523) were diluted in 1% BSA in PBST and incubated with the cells overnight at 4C
Research endpoints include epithelial-barrier integrity, cytoprotective signaling, inflammatory-response markers, peptide biodistribution, and comparisons with parenteral delivery models
From postnatal day 29 onwards, mice were administered a mash made of feed powder and water with or without 2 g ALCAR per kg feed
doi: 10.2337/dc19-0880 18 LiberatiAAltmanDGTetzlaffJMulrowCGtzschePCIoannidisJPAet al
This mechanistic uncertainty limits interpretation of human efficacy data
