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Similarly, 4-methylsulfinylbutyl-GL content remained unchanged in broccoli (cultivar Marathon) heads stored at temperatures of 1 or 4C, 99% relative humidity (RH), for 2, 7, 14, or 28 days, followed by exposure to 8, 15, or 20C, with 99, 90, or 70% RH, respectively, for 3 days (146)

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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection

Prediction of the sequence-specific cleavage activity of Cas9 variants