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Mechanistically, ME suppressed the pathological increase in intracellular Ca 2+ concentration and inhibited calpain activity
At the end of pharmacological treatment, biochemical analyses, immunohistochemistry analysis, and intravital microscopy of the brain microcirculation were carried out to quantify leukocyteendothelium interactions and to assess structural capillary density, astrocyte coverage on cerebral vessels and microglial activation
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