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In the blank cuvette, distilled water (10 l) was added to monobromobimane (stock solution 30 l) in presence of phosphate buffer (3.0 ml)

Vitam Horm 104:153195

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10.1021/bi701559f 95 ShenP.HanL.ChenG.ChengZ.LiuQ

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For the induction of gene expression, 0.050.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG) was added as indicated in different experiments