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Protein extraction and digestion for mass spectrometry For protein extraction and digestion, we used the in-strip protein digestion method, as previously described 21

There is, however, a note that it will last 56 days refrigerated once reconstituted, and up to a year in powdered form

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We then explore the atomic-level conformational dynamics and receptor activation and signalling by fully mapping extracellular peptide binding in the membrane environment, including intracellular Gs protein coupling (which initiates downstream signalling), using long-timescale molecular dynamics (MD) simulations