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The follicles didn't just survive in the lab
V d, mu ca ca bn c nghin cu l nn duy tr mc pH n nh trong khong 7.35 n 7.45
Subsequently, organoids were incubated for 2h in HEPES-saline buffer supplemented with either LPS (10g/mL), MDP (10g/mL), a combination of TNF (10ng/mL) and IFN (50 ng/mL), DCA (30 M) (as physiological positive control), or a mixture of 10 M Forskolin and 10 M 3-isobutyl-1-methylxanthine (F/I, for maximal hormone output)
Its just a temporary digestive response
However, it is critical to recognise that these direct mechanisms are considered secondary, and in some cases remain controversial, compared with the proven impact of indirect pathways
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