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JAMA, 331 (1), 3848

The protein was eluted in wash buffer with 250 mM imidazole and treated with Lamda Phosphatase (New England BioLab) and Alkaline Phosphatase (New England BioLab) overnight at 4 C

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Kervio G, Carre F, Ville NS

Ten microliters of 0.1 mg mL 1 ShHTL7 protein in a reaction buffer containing 20 mM HEPES, pH 7.0, 150 mM NaCl and 80 L of reaction buffer were mixed in a 96-well black plate (Grenier)

Dasari A, Mazard T, Cohen R, Elez E, Tarazona N, Borg C, Rinaudo P, Chne L, Fagerberg J, Schmoll HJ, Cervantes A, Tabernero J, Kopetz S