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selected an optimized n-hexane-ethyl acetate-methanol-water (3:7:4:6, v/v) solvent system for countercurrent chromatography based on partition coefficients (k) and separation factors (), utilizing the lower phase as mobile phase in head-to-tail elution mode, where 400 mg crude extract yielded pure senkyunolide-I (6.4 mg, 98%) and senkyunolide-H (1.7 mg, 93%) in a single run (32)

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53 In the context of necroptosis, therapeutic modulation must be approached with caution