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Then, 30 10 6 cells were collected (representing an estimated coverage of 300), and 30 10 6 cells were plated for treatment the next day with either 5 M 5FU or media only for a further 11 days

Liver paraffin sections (5 mm) were prepared and stained with hematoxylin and eosin (H&E) by the Department of Pathology at the University of Colorado Denver using standard procedures

The sustained GH elevation from DAC already provides continuous stimulation

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