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(1984): DSIP showed analgesic properties in chronic pain patients, with effects mediated through opioid receptor interactions

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The subject population was highly diverse in terms of age, socioeconomic status, education, and geographic neighborhoods throughout the Pacific Northwest, although all participants were Caucasian

Inconsistent signals across plates are most commonly caused by temperature differences between the plate and reagent at the time of addition (ensure both are equilibrated to room temperature for 30 minutes before mixing), insufficient cell lysis (increase mixing time or speed), or signal read outside the stable window (read plates within 10 minutes to 1 hour after stabilization)

DOI: 10.1016/j.jneuroim.2016.08.012 21