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Aliquots of 40 g proteins were then fractionated on 612% SDSpolyacrylamide gels and transferred to PVDF membranes, which were then blocked with 0.1% Tween-20 tris-buffered saline (TBST) solution containing 5% nonfat milk or 5% BSA for 1 h at room temperature
An additional quantity of vehicle was added to the beaker, rinsed, and transferred to the volumetric flask
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This compound is an essential mineral that acts as a cofactor for glutathione and plays an important role in maintaining glutathione activity in the body
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