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This ratio is one of the most reliable biomarkers of cellular redox status, measurable by HPLC or enzymatic assay (Forman et al., Mol Aspects Med 2009)

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Protein isolation and western blotting For standard western blotting, tissues were lysed in RIPA buffer and extracted protein content was determined using the BCA protein assay (Sigma, St

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Fornatora ML, Reich RF, Haber S, Solomon F, Freedman PD

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Prevent glycation: This process occurs when excess the sugars in the body bond to proteins such as collagen, and weakens them

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