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The mice were treated daily with BSO following the approved animal experiment protocol
Sedating antihistamines like diphenhydramine (Benadryl) can be used at night if skin sensitivity is disrupting sleep, but they should not be relied on long-term
A decision on that matter is still pending
For the orthotopic pancreatic cancer model, VRK2-WT or VRK2-KO Pan02 cells in logarithmic phase (8090% confluence) were harvested, detached with 0.25% trypsin-EDTA, centrifuged (1000 rpm, 5 min), and resuspended in cold serum-free RPMI-1640 medium
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Eur J Med Chem 235:114291 Piiril P et al (2010) Matrix metalloproteinases-7, -8, -9 and TIMP-1 in the follow-up of diisocyanate-induced asthma
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