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The rest? Wasted

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Animal experiments and studies of human subjects have shown that IOP-independent factors include oxidative stress, glutamate excitotoxicity, and impaired retinal blood flow 6,7,8

Two essential acidic amino acid residues, such as glutamic acid (Glu) and aspartic acid (Asp), are present in the active site and serve as general bases and acids, respectively (Liang et al., 2022

Interestingly, inhibition of NLRP3 inflammasome activation reversed the M1 polarization of TMAO-stimulated macrophages, suggesting that NLRP3 plays a crucial role as a proteolytic activator in TMAO-induced macrophage responses