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NovaSil clay intervention in Ghanaians at high risk for aflatoxicosis
They believed it enhanced immune function
Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
Heavy metals are considered to be cadmium, copper, lead, copper, nickel and arsenic
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