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(PubMed) De la Fuente M, Hernanz A, Guayerbas N, Victor VM, Arnalich F
If it sat out at room temp for more than 4 hours, toss it
When you are pregnant, "good enough" is not the standard
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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)
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