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There, doctors determined she was suffering from Wernicke's encephalopathy, a neurological condition caused by a deficiency of thiamine, or Vitamin B1, often linked to malnutrition
Protein digestion by trypsin was performed according to the filter-aided sample preparation (FASP) procedure described by Matthias Mann ( Figure 1 2.6 LC-MS/MS analysis Labeled peptides were fractionated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific)
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