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Because high levels of oxidized GSSG are toxic and indicate severe cellular stress, the cell must rapidly recycle it

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The resulting reaction mixture was centrifuged at 15,000 g for 10 min, and the supernatant was analyzed by LC-FL (Waters, Milford, MA, USA) and LC-MS/MS (Shimazu, Kyoto, Japan)

A recent study showed that glucose metabolism was dysregulated and that pyruvate supplementation could prevent neurodegeneration in an animal model of glaucoma 31 , suggesting that ATP production from glycolysis is disrupted, and that other energy fuel pathways, such as -oxidation, may be augmented to compensate for the reduction in energy metabolism

Thus, using younger mice replicates the clinical scenario for a large group of patients