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A Dionex UltiMate 3000 506 LC System (Thermo Scientific) and a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) operated in negative mode was used for targeted measurement
This minimizes movement and reduces the chances of accidental contamination
Yes, the dose and how often B12 shots are given can change
Cai S, Sauve R
Draw the HCG: Insert the needle into the HCG vial, inject the air to prevent a vacuum, invert the vial, and slowly draw out slightly more than your required HCG dose
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